D-Biotin, also known as vitamin H or coenzyme R is a water-soluble member of the B vitamins (B7).
During fatty acid synthesis and gluconeogenesis, biotin acts as a flexible prosthetic group by allowing it to reach different enzymatic active sites.
Biotin can be used in cell culture media for its involvement in a number of cellular processes including gluconeogenesis, synthesis of fatty acids, and amino acids isoleucine and valine.